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1. #FLOWJO DEPARTMENTAL LICENSE SOFTWARE#
2. #FLOWJO DEPARTMENTAL LICENSE PASSWORD#
3. #FLOWJO DEPARTMENTAL LICENSE PLUS#
4. #FLOWJO DEPARTMENTAL LICENSE FREE#

No fee will be charged for the booked hours if the appointment is canceled 24 hours in advance.*Prices are as of 1/1/21 and are subject to change. InstrumentīD FACSAriaII-1/AriaII-3 (BSL2+) Cell SortersīD FACSAriaII-2/AriaII-3 Cell Sorters (Self-Operated) This is important in order to grant everyone access to the machines. Please make sure that you delete time as soon as you know you will not be able to use it.

#FLOWJO DEPARTMENTAL LICENSE PLUS#

The total fee is calculated based on the booked hours plus any actual time used in excess of the booked hours. Instrument fees are based on the following hourly rates billed in 15 minute increments for sorters and in 1 minute increments for analyzers. The trainees will sort their own cells under the supervision and with help of the trainer, if needed.

#FLOWJO DEPARTMENTAL LICENSE SOFTWARE#

Training for cell sorting is provided only to users that are familiar with the Diva software and regularly operate the LSRII, Fortessa and or Canto analyzers.

#FLOWJO DEPARTMENTAL LICENSE FREE#

The training on the FACS analyzers is free of charge.

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To obtain training, please contact the FACS staff at Once trained, you will be issued a username and password to access the online schedule and an account will be set up on the instrument. The online schedule is accessible only to trained users. New users are provided with training before being able to operate the instruments. Instructions and training on the instruments/software For sorting on Aria II-3 please contact the FACS staff at available to each sorter: Use of the BSL2+ sorter is encouraged for clinical samples when testing is uncertain and for cells infected with BSL2+ level pathogens. The AriaII-3 is a BSL2+ level sorter, as it is contained in a Bioprotector cabinet, which maximizes aerosol containment. High-speed cell sorting (staff or self-operated, AriaII-3)

They are also encouraged to initially sort during working hours when FACS staff are present until they have acquired experience with troubleshooting and problems that may arise during sorting. People are encouraged to attend the two training sessions in a short time frame between each other. Training consists of two sessions in which modalities of sorting and instrument set ups are illustrated (see instructions and training section). Trained operators will be able to reserve time directly on the AriaII-2 schedule. High-speed cell sorting (self-operated, AriaII-2) changes in nozzle size, changes in pressure, single cell sorting into 96- or 384- well plates), please include this information in the notes section of the sorting request form and add 30 minutes to your requested sorting time to allow changes from the default setup (NOTE: you will be charged for this special setup time). If your sort requires a special setup (i.e. If the setup notes for your sorting do not indicate special requirements, the AriaII-1 will be set up with a default 85um nozzle 45psi. If you do not receive a confirmation email, your sort has not been scheduled. You will receive a confirmation email when your request has been added to the schedule. An email will be sent to the FACS staff detailing your request and your request will be added to the sorting calendar. To schedule cell sorting on the Aria II-1, go to the online schedule, choose the time you would like to begin and end, click on REQUEST and fill out the pop-up window with your lab and sort information. Requests for cell sorting can be made using the online schedule or by contacting the FACS Staff by email at The online schedule is accessible only to Washington University researchers and requires a username and password. High-speed cell sorting (staff-operated, AriaII-1)Īppointments with staff are available Monday through Friday from 10am to 5pm. Please note that each user needs to have an individual account. First time users: please register here. When you log in fill in all required information and request access to instruments. Please allow one business day for the activation of your account. Contact the FACS Staff if you have any questions or problems with the new account at and pricing Service available to: All entities, including for-profit organizations.

Washington University Medical Directorships.Cytogenetics and Molecular Pathology (CMP) Laboratory.